FastQCFastQC Report
Tue 28 May 2019
HGHW5BGXB_n01_HFH04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHW5BGXB_n01_HFH04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9015456
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC989971.098080895741713No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG763110.8464463694348905No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG527510.5851173806405355No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA479180.531509443338196No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC474630.526462554972261No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG438430.48630928929163425No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT341810.3791377829363262No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC203680.22592312579641005No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT189300.20997274014758655No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA180890.20064431571736363No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG179690.19931326823623788No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA160820.17838254659553548No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC140780.15615405366073554No Hit
TCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCAT137050.15201671440690298No Hit
CTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATC126230.14001510295208586No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT116700.12944436753947886No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG110250.12228998732842797No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA96250.1067611000486276No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT674450.069.499998
AGTAGGT682350.069.4039467
TAGGTTG675600.069.3659
TAGTAGG677100.069.34236
AGGTTGT713650.069.3300310
GGTAGTA906550.069.323684
GTTGTAT506750.069.2860212
GAGGTAG956050.069.216082
TGAGGTA933150.069.127071
TATAGTT553250.069.11960616
GTAGTAG905900.069.092135
GCTACAT235500.069.0522
TGTATAG606100.069.0045814
TGAGACC544400.068.8338245
GTGTGGT209250.068.8281115
CAGCACG174900.068.8024754
GTTGTGT212550.068.79536412
TTGTATA607500.068.781813
CTGAGAC545750.068.7589954
GGTTGTG212900.068.74802411
Produced by FastQC (version 0.11.5)