FastQCFastQC Report
Tue 28 May 2019
HGHW5BGXB_n01_HFH03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHW5BGXB_n01_HFH03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6669118
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG625990.9386398621226975No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC543720.8152802214625683No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG439580.6591276387672252No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA382660.5737790214538114No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG378650.5677662323563626No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC319940.4797336019545613No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT272580.40871971376125005No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA132770.19908179762301403No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC130920.1963078176154628No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC117440.17609524977665714No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT105570.15829679426874738No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA100290.15037970538233092No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG99280.14886526224307323No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT88220.1322813601438751No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG78310.11742182399531692No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG72880.1092798178109909No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC72630.10890495564780829No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA71930.10785534159089703No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT595600.069.53288
AGTAGGT602500.069.5037847
TAGGTTG595700.069.45659
TAGTAGG596550.069.43476
GTTGTAT451850.069.4016512
GGTAGTA797650.069.38954
AGGTTGT633600.069.31258410
TATAGTT495750.069.3012216
TGAGGTA816300.069.293741
GTAGTAG796750.069.257015
GAGGTAG844350.069.191342
AGACCCT497400.069.125047
TGTATAG543500.069.1109614
GCTACAT176800.069.0535662
TTGTATA543600.069.0323113
GTGTGGT181650.068.959715
GTTGTGT184550.068.9550212
CTGAGAC500550.068.954934
TGAGACC498800.068.944245
GAGACCC499850.068.8484346
Produced by FastQC (version 0.11.5)