FastQCFastQC Report
Tue 28 May 2019
HGHW5BGXB_n01_HFH02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHW5BGXB_n01_HFH02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9504986
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC886630.9328051614173867No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG742550.7812215609786275No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG540590.5687436046723268No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC376980.3966128934855874No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA370040.3893114624261414No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG344540.36248343763999236No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT252630.265786819675484No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT249560.2625569359071123No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC196910.2071649553192398No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA161810.17023696826065815No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG159030.16731218751926621No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA137950.14513435369604963No Hit
TCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCAT109200.11488707084892076No Hit
CTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATC107700.11330895174385318No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT706450.069.3486568
AGTAGGT714400.069.327227
AGGTTGT746050.069.2842210
GTTGTAT553300.069.2832412
GGTAGTA966500.069.247024
TAGGTTG706800.069.239679
TAGTAGG708750.069.219056
TGAGGTA989700.069.208591
CAGCACG219050.069.141054
TATAGTT614650.069.1229516
GAGGTAG1019600.069.094192
GTAGTAG966000.068.928135
TTGTATA674650.068.8882113
TGTATAG675700.068.880314
CTGAGAC604100.068.874074
GCTACAT220050.068.842752
AGACCCT603350.068.803427
GTTGTGT198150.068.7616212
AGCTTAT224300.068.7555162
GTGTGGT193950.068.7543415
Produced by FastQC (version 0.11.5)