FastQCFastQC Report
Tue 28 May 2019
HGHW5BGXB_n01_HFH01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHW5BGXB_n01_HFH01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8620043
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG689400.7997639918965601No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC556580.6456812338407129No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG405290.4701716685171988No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA387400.44941771172139167No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG385740.44749196726744866No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC325820.3779795530022298No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT268900.3119473997983537No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG169220.19630992560013913No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATCA132600.15382753891134882No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC125290.14534730279187702No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA124780.14475565841144877No Hit
TCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCAT108250.1255794199634503No Hit
CTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGGCACCATC102400.11879291089383197No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT99840.11582308812148616No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT607600.069.360328
AGTAGGT614500.069.356117
GGTAGTA828100.069.220224
GTTGTAT471350.069.2176412
TAGTAGG609900.069.200736
AGCTTAT202300.069.1893842
AGGTTGT643650.069.1727910
TAGGTTG608700.069.171339
TATAGTT523150.069.1567516
TGAGGTA851050.069.154141
GAGGTAG875950.069.123492
GCTACAT227950.069.1119842
CTGAGAC527650.068.9076464
GTAGTAG828650.068.89755
AGACCCT526400.068.869417
TGAGACC527100.068.835465
TGTATAG576050.068.8019914
GCTTATC203200.068.796423
CGTAAAT178500.068.764039
TTGTATA576250.068.7279213
Produced by FastQC (version 0.11.5)