FastQCFastQC Report
Sun 22 Jan 2017
HGHTTBGX2_n02_mt_atac_23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHTTBGX2_n02_mt_atac_23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61425131
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG665340.1083172293112407No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAG1688650.023.70439369
GAGTGTA2326000.021.3775369
GTGTAGA1182150.020.76707869
CGAGTGT3142050.017.58680369
TATCCCG182200.017.1781125
GTATTAT223550.016.422361
AGGACTC174550.015.52174955
TATACCG94700.014.9235395
ATCCCGT193400.014.8468136
TACCCCG196300.014.6272515
AAGGGTC259450.014.09140615
TATATAG341500.014.0503295
CCTTATA305000.013.9908012
GGGTACA213650.013.8918751
ACGAGTG3992250.013.84331468
CTAGTAC61850.013.7092583
CTATACT73050.013.63647654
TGTACAG220350.013.6095635
AGGATAG273850.013.52149928
GAGAGCG228000.013.4863417
Produced by FastQC (version 0.11.2)