FastQCFastQC Report
Sun 22 Jan 2017
HGHTTBGX2_n02_mt_atac_22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHTTBGX2_n02_mt_atac_22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63596935
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG771140.12125427113743768No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAG2999550.024.43340769
GTGTAGA2000450.023.51006169
GAGTGTA4194000.020.99524169
TATCCCG164000.018.4330795
AGGACTC166650.018.0365455
GTATTAT180400.017.4079061
TGTAGAT1445400.016.99959469
CGAGTGT5607550.016.98512569
TACCCCG192700.016.5642385
TATATAG294800.016.521785
GAGAGCG215850.016.3538937
TGTACAG214300.016.342325
AAGGGTC247450.016.32172615
AGGATAG244850.016.20786328
ATAGAGG261800.016.0388328
TCGGTGG381400.016.013269
CCTTATA281950.015.966092
GGGTACA206150.015.7685971
TCTCGGT425050.015.63514469
ATCCCGT182650.015.60755356
Produced by FastQC (version 0.11.2)