FastQCFastQC Report
Sun 22 Jan 2017
HGHTTBGX2_n02_mt_atac_07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHTTBGX2_n02_mt_atac_07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79341279
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1621720.20439801581721415No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTA263700.026.7962175
TCGGTGG951150.024.91527469
CCGCTAT282400.024.91057422
GAACGCG280200.024.09760923
AGGGTTG180650.023.7553335
CGAAACG289150.023.62918314
ACACCGT257400.023.368156
GGGATAT223450.023.3255316
GACTCGG292350.023.262776
TACGTCT307200.023.1359964
AACGGAG292450.023.0956617
CCAACCG306500.023.06160718
GCTATTC306350.022.99851424
ACCGTGG257150.022.97838
CCGCTTA322800.022.91233318
CCGTGGA255200.022.8174389
GTGTACG317700.022.8070491
TGTTCCG336450.022.7540045
GTCTACA310650.022.6928337
GCACCGA332450.022.6341846
Produced by FastQC (version 0.11.2)