FastQCFastQC Report
Sun 22 Jan 2017
HGHTTBGX2_n02_mt_atac_05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHTTBGX2_n02_mt_atac_05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74529763
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8017841.0757903523723804No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTGG443100.044.4419769
CTCGGTG723200.027.20088668
CCGCTCG388450.025.924759
GGGTCTT456900.025.15670817
ACATCCG394300.025.124215
TGTACAG332500.025.0355955
TAGAGGA465750.024.9012559
GAAGGGT463750.024.84773314
TATCCCG293250.024.7779315
TTGTACA339400.024.5768744
TATATAG483700.024.3857655
ACTTGTA338150.024.2905082
AGCGCAC376700.024.0535910
CGCTTAC379250.023.98702419
TATAGAG485550.023.9185127
CGAGGGA374250.023.84435816
AAGGGTC482750.023.83466315
GCCTCCC409600.023.79526722
GAGAGCG380900.023.7476147
ATAGAGG491300.023.6822668
Produced by FastQC (version 0.11.2)