FastQCFastQC Report
Sun 22 Jan 2017
HGHTTBGX2_n02_mt_atac_03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHTTBGX2_n02_mt_atac_03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65869545
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3638130.5523235358616793No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTGG733150.029.87162669
CTCGGTG1100100.028.93459769
GAACGCG195750.026.61489523
CGAAACG210800.025.39292114
ACGTCTA185850.025.3911365
GACTCGG212250.025.1566166
GCACCGA229750.024.7707986
ACCGATC242450.023.8171028
CGATCGC237750.023.44955410
CGCACCG236200.023.4080755
GAGAACG227800.023.24689721
CGCGGGT219850.023.19731726
CGGGTCT218100.022.94236228
AACGGAG233550.022.80556317
CCGCTTA224650.022.71135318
AGCGTCG206300.022.61468312
GGTCGAA242550.022.50394611
TTGAGCG215050.022.4281449
GTGTACG221350.022.410721
CCGCTAT210000.022.37645122
Produced by FastQC (version 0.11.2)