FastQCFastQC Report
Sun 22 Jan 2017
HGHTTBGX2_n02_mt_atac_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHTTBGX2_n02_mt_atac_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71584748
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2412510.33701452717274355No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTGG352300.040.5094169
CTCGGTG555100.027.02131369
TATCCCG238500.020.363815
CCGCTCG307400.020.1314149
TGTTCCG336200.020.05435
CGCTTAC328450.019.24204819
TCTCGGT832050.018.91881269
TACCCCG329950.018.8775845
CGACCCT339650.018.86061110
ACATCCG323100.018.84045
TCCGACC357250.018.672298
AGCGCAC277900.018.63472710
AGTGTAG4286300.018.56773469
TGTACAG262500.018.4100555
CCGACCC367500.018.3776879
GTTCCGA360850.018.3695956
ATCCCGT251100.018.2579466
GCCGCTT348900.018.2418417
TTCCGAC361900.018.0409227
CCTGCCG354350.018.01618414
Produced by FastQC (version 0.11.2)