FastQCFastQC Report
Sun 22 Jan 2017
HGHTTBGX2_n01_mt_atac_23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHTTBGX2_n01_mt_atac_23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61425131
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGCA1008950.024.47052869
ACTAGGC1696900.024.12694469
GACTAGG2336650.021.77785769
TAGGCAT700950.017.89212469
AGACTAG3161500.017.62308769
TATCCCG174000.017.5635055
AGGACTC184450.017.2229565
ATCTCGT167600.016.88510969
GTATTAT217200.016.6340831
TATACCG94450.016.1050995
TACCCCG204800.015.4947515
ATCCCGT185800.015.2228376
TGTACAG222450.014.9320915
GGACTCC278100.014.4618886
TACACAG87900.014.3621345
GAGAGCG234350.014.3492287
GGGTACA209950.014.3321921
CGAGGGA208400.014.28333316
ACTCCTC229500.014.171598
CCTCCCT200400.014.11267311
Produced by FastQC (version 0.11.2)