FastQCFastQC Report
Sun 22 Jan 2017
HGHTTBGX2_n01_mt_atac_22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHTTBGX2_n01_mt_atac_22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63596935
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT192150.028.37773569
CCGTACT1910750.024.80404969
ACCGTAC2959500.024.7671969
CGTACTA1159250.021.22030469
GACCGTA4251500.020.95840669
AGGACTC168850.019.5907215
TATCCCG160550.018.3906175
GTATTAT181200.017.4826491
TGTACAG204600.017.3814475
AGATCTC362250.017.34852869
AGGGGGA170850.017.225429
GTACTAG814800.016.99303269
AGACCGT5701100.016.80859269
TACCCCG198350.016.7640025
TATATAG290600.016.7005715
ACTCCTC206550.016.6647828
CCTCCCT179750.016.65508311
ACTTGTA162800.016.5474412
CTTGTAC215000.016.3802493
CGAGGGA195150.016.3311916
Produced by FastQC (version 0.11.2)