FastQCFastQC Report
Sun 22 Jan 2017
HGHTTBGX2_n01_mt_atac_07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHTTBGX2_n01_mt_atac_07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79341279
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT541000.039.16917469
TATGCCG161100.038.61263743
CGTATGC170750.036.51309241
ATGCCGT176350.034.6272344
CCGTCTT176900.034.53833447
GTATGCC196200.032.49662842
GCCGTCT190600.031.8379346
TGCCGTC219150.028.33633245
TCTCGTA214600.028.31331338
AGGGTTG187550.027.2923895
ACGTCTA256550.026.351715
GCTTGAA233750.025.3606156
CCGCGGG231750.025.03194212
GATAGGG198950.025.017812
ACACCGT260350.025.0132226
TGCTTGA234400.024.9808555
GGGATAT235250.024.70457816
ACGGTTA300150.024.52836827
GCGGGAT238000.024.41834314
ATACGGT304700.024.34185425
Produced by FastQC (version 0.11.2)