FastQCFastQC Report
Sun 22 Jan 2017
HGHTTBGX2_n01_mt_atac_05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHTTBGX2_n01_mt_atac_05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74529763
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG4744560.6365993676915356RNA PCR Primer, Index 27 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG562750.061.54670369
CCGTCTT803850.058.5705947
GCTTGAA808250.057.6025556
GCCGTCT821150.057.20121846
CTTGAAA819600.056.976457
AAAGGGG615200.056.19841468
TATGCCG850400.055.7921243
TCGTATG849150.055.78785740
CGTATGC852650.055.78126541
ATGCCGT858000.055.14062544
CTCGTAT851000.054.84512339
TGCCGTC870100.054.29755845
TCTCGTA861000.053.93140438
GTATGCC893250.053.5493242
TGAAAAA1025450.046.68443759
TTCTGCT1055050.043.8165952
GAAAAAA1124500.042.72301560
ATCTCGT1133700.041.13269837
TGCTTGA1192550.038.88666555
AATGGGG149300.035.06591468