FastQCFastQC Report
Sun 22 Jan 2017
HGHTTBGX2_n01_mt_atac_03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHTTBGX2_n01_mt_atac_03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65869545
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG179200.046.2253143
CGTATGC175700.045.4796341
GTATGCC206550.041.1238642
ATGCCGT182850.040.62276544
GCCGTCT191750.037.90851246
CCGTCTT214600.035.81764247
TGCCGTC217250.035.6191345
TCTCGTA212700.032.6701938
ATCTCGT408050.028.1131169
GCGTATG80700.026.50754740
GAACGCG194200.026.16522623
AGCGTCG220050.025.9870712
CATCTCG698700.025.96620669
TGCTTGA281950.025.87065555
TTGAGCG229600.025.7172819
GCTTGAA295300.025.36735256
GACTCGG212600.025.0181276
AAGGGGG373200.024.66281569
CGAAACG214100.024.56696914
ACGTCTA185350.024.4718345
Produced by FastQC (version 0.11.2)