FastQCFastQC Report
Sun 22 Jan 2017
HGHTTBGX2_n01_mt_atac_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHTTBGX2_n01_mt_atac_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71584748
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG980890.1370249986770925RNA PCR Primer, Index 17 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG249600.042.47602543
CGTATGC255500.041.8074941
ATGCCGT251950.041.7372144
GCCGTCT250050.041.61167546
TGCCGTC258200.040.8064445
TCGTATG267000.039.6321140
GTATGCC278900.038.65733742
CTCGTAT271250.036.7353139
CCGTCTT293950.035.71455847
AAGGGGG222050.035.06360269
TCTCGTA314650.031.9862138
GCTTGAA367350.028.07587856
AAAGGGG306550.025.45452968
ATCTCGT495650.024.09179969
CTTGAAA456600.022.80656657
AGCGCAC282050.021.52177210
TATCCCG244700.020.8334885
CGCACGA295700.020.38835512
TGTACAG260950.020.1045075
TACCCCG323950.020.0277585
Produced by FastQC (version 0.11.2)