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        Note that additional data was saved in multiqc_data when this report was generated.


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        If you use plots from MultiQC in a publication or presentation, please cite:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        About MultiQC

        This report was generated using MultiQC, version 1.0.dev0

        You can see a YouTube video describing how to use MultiQC reports here: https://youtu.be/qPbIlO_KWN0

        For more information about MultiQC, including other videos and extensive documentation, please visit http://multiqc.info

        You can report bugs, suggest improvements and find the source code for MultiQC on GitHub: https://github.com/ewels/MultiQC

        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        Report generated on 2018-12-07, 20:12 based on data in: /beegfs/mk5636/logs/html/HGHK3BGX9/merged


        General Statistics

        Showing 30/30 rows and 3/5 columns.
        Sample Name% Dups% GCM Seqs
        HGHK3BGX9_n01_AHm17
        49.4%
        49%
        39.7
        HGHK3BGX9_n01_AHm18
        42.9%
        49%
        23.1
        HGHK3BGX9_n01_AHm19
        44.9%
        49%
        22.0
        HGHK3BGX9_n01_AHm20
        44.7%
        49%
        22.6
        HGHK3BGX9_n01_AHm21
        50.7%
        48%
        21.9
        HGHK3BGX9_n01_AHm22
        49.7%
        49%
        38.4
        HGHK3BGX9_n01_AHm23
        52.6%
        48%
        20.9
        HGHK3BGX9_n01_AHm24
        49.6%
        49%
        10.7
        HGHK3BGX9_n01_AHm25
        56.8%
        49%
        14.4
        HGHK3BGX9_n01_AHm26
        49.8%
        49%
        26.9
        HGHK3BGX9_n01_AHm27
        50.5%
        49%
        35.4
        HGHK3BGX9_n01_AHm28
        39.7%
        49%
        18.3
        HGHK3BGX9_n01_AHm29
        44.4%
        49%
        19.2
        HGHK3BGX9_n01_AHm30
        44.3%
        49%
        21.0
        HGHK3BGX9_n01_undetermined
        72.5%
        45%
        17.0
        HGHK3BGX9_n02_AHm17
        48.2%
        49%
        39.7
        HGHK3BGX9_n02_AHm18
        41.4%
        49%
        23.1
        HGHK3BGX9_n02_AHm19
        43.4%
        49%
        22.0
        HGHK3BGX9_n02_AHm20
        43.1%
        49%
        22.6
        HGHK3BGX9_n02_AHm21
        48.9%
        48%
        21.9
        HGHK3BGX9_n02_AHm22
        48.5%
        49%
        38.4
        HGHK3BGX9_n02_AHm23
        50.6%
        48%
        20.9
        HGHK3BGX9_n02_AHm24
        47.0%
        49%
        10.7
        HGHK3BGX9_n02_AHm25
        52.2%
        49%
        14.4
        HGHK3BGX9_n02_AHm26
        47.8%
        49%
        26.9
        HGHK3BGX9_n02_AHm27
        48.9%
        49%
        35.4
        HGHK3BGX9_n02_AHm28
        38.8%
        49%
        18.3
        HGHK3BGX9_n02_AHm29
        42.8%
        49%
        19.2
        HGHK3BGX9_n02_AHm30
        43.1%
        49%
        21.0
        HGHK3BGX9_n02_undetermined
        69.7%
        45%
        17.0

        Demultiplexing Report

        Demultiplexing Report
        Total Read Count: Total number of PF (Passing Filter) reads in this library.
        Portion: The proportion of reads that represent the individual library in the entire Library Pool.

        Showing 15/15 rows and 2/2 columns.
        Sample NameTotal Read CountPortion (%)
        undetermined_library
        17,036,662
        4.8
        AHm17
        39,669,932
        11.3
        AHm18
        23,117,509
        6.6
        AHm19
        21,951,244
        6.2
        AHm20
        22,577,489
        6.4
        AHm21
        21,874,632
        6.2
        AHm22
        38,403,791
        10.9
        AHm23
        20,916,554
        6.0
        AHm24
        10,681,431
        3.0
        AHm25
        14,381,104
        4.1
        AHm26
        26,850,397
        7.6
        AHm27
        35,401,299
        10.1
        AHm28
        18,309,204
        5.2
        AHm29
        19,160,888
        5.5
        AHm30
        21,024,966
        6.0

        Run Statistics

        Run Statistics

        Showing 1/1 rows and 4/4 columns.
        Sample NameTotal # of Single-End ReadsTotal # PF Reads% Undetermined % PhiX Aligned
        4.0
        368,687,296
        351,357,102
        4.8
        3.2

        FastQC

        FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

        Sequence Quality Histograms

        The mean quality value across each base position in the read. See the FastQC help.

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        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality. See the FastQC help.

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        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called. See the FastQC help.

        Click a heatmap row to see a line plot for that dataset.

        rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content. See the FastQC help.

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        Per Base N Content

        The percentage of base calls at each position for which an N was called. See the FastQC help.

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        Sequence Length Distribution

        All samples have sequences of a single length (101bp).


        Sequence Duplication Levels

        The relative level of duplication found for every sequence. See the FastQC help.

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        Overrepresented sequences

        The total amount of overrepresented sequences found in each library. See the FastQC help for further information.

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        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. See the FastQC help. Only samples with ≥ 0.1% adapter contamination are shown.

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