FastQCFastQC Report
Fri 7 Dec 2018
HGHK3BGX9_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHK3BGX9_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17036662
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1212980.7119821946341367No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCCTA44850.028.93007520-21
TACAGTT52650.024.93838724-25
CCGTATA7300.022.40479360-61
GGTGCCT60050.022.19622418-19
ATGGTGC60350.021.88251316-17
TGGTGCC70900.018.96673818-19
ATTGATG68900.018.88676812-13
CGTATAA10800.014.92765862-63
TCGTGTA52950.014.36808714-15
ACAGTTT57200.013.99112826-27
TAGCCGT8900.013.58257558-59
GTAGGGA54900.013.30559218-19
CGGGTAT5603.914647E-812.0704261
AGCGTCG62200.012.0464410-11
TACCGTA5554.271169E-811.9950399
TCCGATC10650.011.7870831
GTGTAGG62000.011.77818316-17
CCGATCT12000.011.2562052
AGCCGTA12500.011.18778158-59
GAGTGTA31800.011.12603328-29
Produced by FastQC (version 0.11.5)