FastQCFastQC Report
Fri 7 Dec 2018
HGHK3BGX9_n02_AHm30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHK3BGX9_n02_AHm30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21024966
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA20050.016.1022959
CGTATCA28000.012.29805862-63
CTACCGT28050.011.8474928
GCCGTAT28900.011.74907860-61
CGGGGAT59250.010.8389381
CGCCGTA37550.010.05313858-59
AAGGGGG212750.09.58926182-83
CGGGGTT69350.09.5347581
ACTACCG37450.09.3803367
TAGGGGG76200.09.31348850-51
CGAGAAT47850.08.748671
CGCGGGT39500.08.6711491
AAAAGGG241750.08.54641580-81
TCGCCGT44200.08.16461658-59
TCGCGTT14150.08.05094430-31
AGGGGGG228250.07.96926182-83
CGCGGAT27500.07.95731261
CGGGAAT50900.07.8505991
AAAGGGG219550.07.818698480-81
GACGCTA23400.07.7105944
Produced by FastQC (version 0.11.5)