FastQCFastQC Report
Fri 7 Dec 2018
HGHK3BGX9_n02_AHm29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHK3BGX9_n02_AHm29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19160888
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA33450.021.79761362-63
GCCGTAT36300.020.21365760-61
CGCCGTA42350.017.65969858-59
TACCGTA20900.016.3557689
CCGTATC51050.014.65236860-61
TCGCCGT50550.014.4662458-59
TGGTCGC50850.014.4250854-55
TAGGGGG89650.014.3765450-51
AAGGGGG260050.014.09845282-83
AAAAGGG279850.013.0578180-81
CTACCGT27550.012.5794328
AAAGGGG257350.011.65888380-81
GTCGCCG68400.011.00232656-57
GTAGATC71000.010.36267644-45
ACTACCG34500.010.31996257
GTATCAT74650.010.21273262-63
TTCGCCT74950.09.94293634-35
GTGGTCG77600.09.94196854-55
TCTCGGT72800.09.58525848-49
AGGGGGG336800.09.52321982-83
Produced by FastQC (version 0.11.5)