FastQCFastQC Report
Fri 7 Dec 2018
HGHK3BGX9_n02_AHm28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHK3BGX9_n02_AHm28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18309204
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA18550.013.0531119
CTACCGT23350.011.1825258
CGACCGT15650.010.6388081
ACTACCG29350.010.0278377
CGGGGTT61800.09.6988741
CGGGTAT19100.09.21526051
GGGGAAT95200.09.1443621
CGGGGAT56650.09.06907751
CGCGGGT35000.08.8345531
CGGGAAT43300.08.7890391
CTCGTAT18700.08.6492121
GGGGGAT95100.08.6037381
CCGGGAT51450.08.506321
GATCGGA191850.08.45668694-95
GTCCGAT14305.456968E-128.316551
CTCGGTT36100.08.3018061
ATCGGAA183650.08.21251194-95
CGACCTA11001.4786565E-88.201334
AGATCGG224450.07.90672994-95
GCGTCGT75850.07.77810494-95
Produced by FastQC (version 0.11.5)