FastQCFastQC Report
Fri 7 Dec 2018
HGHK3BGX9_n02_AHm27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHK3BGX9_n02_AHm27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35401299
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT56300.018.64078160-61
CGTATCA55050.018.42024262-63
TACCGTA38400.017.6805119
CGCCGTA69450.015.51958658-59
CTACCGT50700.014.7013698
TCGCCGT80600.012.90136258-59
CCGTATC82050.012.73281260-61
TGGTCGC87900.012.34141554-55
AAGGGGG418300.011.6013882-83
ACTACCG66750.010.8104027
AAAGGGG427650.010.80750680-81
AGGGGGG487800.010.30915582-83
AAAAGGG469850.010.23150680-81
TAGGGGG140750.010.168735550-51
CGGGGAT100000.010.1325421
CGCGGGT62700.010.0148671
CGCGATT12400.09.9744841
GTCGCCG118050.09.27031956-57
CGGGTAT36550.09.1106521
GATAGGG122850.08.90620236-37
Produced by FastQC (version 0.11.5)