FastQCFastQC Report
Fri 7 Dec 2018
HGHK3BGX9_n02_AHm26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHK3BGX9_n02_AHm26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26850397
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA46600.021.35321462-63
GCCGTAT48300.021.04048360-61
TACCGTA30200.020.9081139
CGCCGTA60250.017.37723758-59
CTACCGT39300.016.6699398
TCGCCGT66150.015.10954758-59
TGGTCGC68950.014.73510654-55
CCGTATC73750.014.06949460-61
CGCGTAT5301.549779E-913.4631891
AAAGGGG378300.013.29903780-81
GGGTTAA93050.013.22480966-67
ACTACCG49100.012.6654887
TAGGGGG117350.012.66149250-51
AGGGGGG472900.012.40929482-83
CGCGGGT48950.012.0504021
GTCGCCG94500.011.12856556-57
AAGGGGG375450.010.95633482-83
CCGTATG35750.010.42495710-11
GTATCAT101200.010.39581162-63
TCTCGGT100100.010.38525348-49
Produced by FastQC (version 0.11.5)