FastQCFastQC Report
Fri 7 Dec 2018
HGHK3BGX9_n02_AHm25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHK3BGX9_n02_AHm25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14381104
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATC593380.412610881612427Illumina Single End PCR Primer 1 (97% over 35bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG156280.10867037746198067No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA77850.040.57249562-63
GCCGTAT82350.039.44332560-61
CGCCGTA86450.037.1830558-59
TCGCCGT88300.036.0813858-59
TGGTCGC90000.035.94828454-55
CCGTATC99100.031.84214660-61
GTAGATC100800.031.0539644-45
TCGGTGG105200.030.52541750-51
GTGGTCG107350.030.04984754-55
TACCGTA17750.029.4215169
TAGTGTA113000.029.27559740-41
GTCGCCG111000.029.08482456-57
CGGTGGT113750.028.54495652-53
TAGGGGG193200.028.4764550-51
CTCGGTG108750.028.39406850-51
GGCTATA116850.027.96546734-35
TCTCGGT110800.027.78164148-49
GGTCGCC118400.027.4073756-57
TAGATCT114600.027.29377244-45
GCTATAG118900.027.10403836-37
Produced by FastQC (version 0.11.5)