FastQCFastQC Report
Fri 7 Dec 2018
HGHK3BGX9_n02_AHm24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHK3BGX9_n02_AHm24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10681431
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATC127450.11931921855788798Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA24350.028.47778162-63
GCCGTAT26150.027.60291760-61
ACGTGTA28900.023.81635940-41
CGCCGTA31850.022.66039358-59
TGGTCGC31550.022.42105954-55
TCGCCGT32550.021.73544758-59
TAGGGGG76500.021.50233550-51
TACCGTA14200.020.3944859
GACATAA55400.019.6253455
GTAGATC36450.018.62296144-45
AAAAGGG210600.018.45621580-81
AAGGGGG217650.018.11075882-83
CGACCTA3951.8189894E-1118.031954
CAGTACG37800.017.89498336-37
AAAGGGG197000.017.6185480-81
CTACCGT15650.017.5940828
CCGTATC40150.017.50488560-61
GTACGTG41900.016.99348438-39
TACGTGT41500.016.92851338-39
AGACATA69500.016.4657214
Produced by FastQC (version 0.11.5)