FastQCFastQC Report
Fri 7 Dec 2018
HGHK3BGX9_n02_AHm23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHK3BGX9_n02_AHm23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20916554
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA33100.025.46989462-63
CGCCGTA42800.021.13445758-59
TCGCCGT47500.018.24354258-59
TGGTCGC49700.017.71986654-55
GCCGTAT50650.017.1577160-61
TACCGTA19550.016.9991939
CCGTATC52650.016.32555260-61
TAGGGGG104800.015.98944350-51
GTACGTC53250.015.95595232-33
TACGTCC53150.015.89666732-33
AAGGGGG330100.015.57277582-83
GTGTACG57550.014.68147530-31
AAAAGGG353400.014.47798780-81
CTACCGT24450.013.591468
GTATCAT66200.013.48828162-63
AAAGGGG286800.013.33028280-81
ACGTCCT63500.013.26786234-35
GTCGCCG66950.013.19089456-57
TGTACGT67550.013.07021130-31
GTAGATC66200.012.94198144-45
Produced by FastQC (version 0.11.5)