FastQCFastQC Report
Fri 7 Dec 2018
HGHK3BGX9_n02_AHm22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHK3BGX9_n02_AHm22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38403791
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA39050.014.8326699
CTACCGT51850.012.1773848
CGGGGAT122150.010.6316091
CGCGGGT72800.010.3895211
AAGGGGG396950.010.33293982-83
CGTATCA48750.010.18119462-63
GCCGTAT50400.010.17608460-61
ACTACCG68500.010.1179897
CTCGGTT75900.09.4011131
CGGGGTT128750.09.3846111
CGCGATT12900.09.218921
TAGGGGG136000.08.98783150-51
CGCCGTA60900.08.84927758-59
AAAAGGG459300.08.81066180-81
CCCGTAT58600.08.5235691
CTCGAAT85300.08.4208841
CGTATCG40300.08.1322073
CTCGGAT106750.08.065671
CGGGTAT40500.07.9871
CGCGGAT49800.07.7372291
Produced by FastQC (version 0.11.5)