FastQCFastQC Report
Fri 7 Dec 2018
HGHK3BGX9_n02_AHm21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHK3BGX9_n02_AHm21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21874632
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA29500.021.57559462-63
TACCGTA19950.020.2283929
CGCCGTA39550.016.92807858-59
TAGGGGG107200.016.7606950-51
CTACCGT24450.016.1157238
AAGGGGG332150.015.46270282-83
AAAAGGG358200.014.47675180-81
CCGTATC45700.014.34035660-61
GCCGTAT47150.013.94970860-61
TGGTCGC47500.013.89277954-55
TCGCCGT46100.013.801958-59
CGCAAAA44000.013.0812421
CGAATCG26050.012.7624933
ACTACCG33550.012.3096737
AAAGGGG290200.012.15023980-81
AATCGAC30100.011.3554845
CGACCTA8258.185452E-1110.9344244
GCAAAAG188000.010.7281371
CGTATGG17850.010.63923912-13
CCCTCAT192550.010.5041844
Produced by FastQC (version 0.11.5)