FastQCFastQC Report
Fri 7 Dec 2018
HGHK3BGX9_n02_AHm20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHK3BGX9_n02_AHm20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22577489
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA24700.013.2629529
GCCGTAT31050.012.38825560-61
AAGGGGG277100.011.45432682-83
CTACCGT29250.010.8743758
CGTATCA29250.010.63591362-63
CGCCGTA38900.010.61970758-59
AAAAGGG298450.010.57850680-81
TAGGGGG91750.09.77884450-51
CGTATCG25050.09.291923
CGCTAAT9357.86531E-99.1586841
CGCGGGT40300.09.0898731
CCGGGAT62550.08.8987851
CGGGGAT66750.08.7664961
GTATCGA30700.08.6614894
CTCGGAT62650.08.6567711
ACTACCG40850.08.5998367
CCGTGTA49800.08.48304140-41
CGGGGTT75650.07.92380481
TGGTCGC49800.07.817402454-55
GGGGGGA302150.07.777239320-21
Produced by FastQC (version 0.11.5)