FastQCFastQC Report
Fri 7 Dec 2018
HGHK3BGX9_n02_AHm19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHK3BGX9_n02_AHm19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21951244
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA21500.014.1324859
CGTATCA27500.011.4856162-63
AAGGGGG238450.010.65952982-83
GCCGTAT31650.010.35296560-61
CGGGGAT70600.010.3103441
TAGGGGG83650.010.10157550-51
CTACCGT30300.09.8706848
CGGGAAT53050.09.8649231
AAAAGGG266100.09.65841280-81
CGCGGGT42500.09.4032231
CTCGGAT64100.09.0549951
ACTACCG38850.08.5533567
CGCCGTA38650.08.53820958-59
CGGGGTT70000.08.2917871
AAAGGGG245200.08.29030580-81
CTCGTTT43550.08.1933121
CGTATCG26750.08.1680233
AGGGGGG266100.08.06864482-83
GGGGGGA255450.07.907145520-21
GTATCGA34400.07.8675764
Produced by FastQC (version 0.11.5)