FastQCFastQC Report
Fri 7 Dec 2018
HGHK3BGX9_n02_AHm18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHK3BGX9_n02_AHm18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23117509
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA21000.011.9825189
CGCGGGT43700.011.7564591
CGGGTAT23500.09.5140231
CGGGAAT51400.08.9772391
CGCGTAT4300.008091638.8502531
GGGGAAT124450.08.753361
CGGGGAT67000.08.7330211
CTACCGT28350.08.707798
CGACCGT19300.08.6267041
CACGAAT30700.08.212431
CTGGAAT230550.08.1295271
CGGGAAC80300.07.9974661
CGGGCAT93400.07.94532871
CTCGGAT61400.07.82505131
AGGGGGG249400.07.798311782-83
CCGGAAT58000.07.7916561
GCCGTAT30000.07.75641860-61
TCGGATT38000.07.7554542
AAGGGGG221500.07.696059780-81
CTCGGTT42650.07.69598531
Produced by FastQC (version 0.11.5)