FastQCFastQC Report
Fri 7 Dec 2018
HGHK3BGX9_n02_AHm17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHK3BGX9_n02_AHm17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39669932
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA41300.011.3808369
CGGGGAT115700.010.1557761
CTCGTAT40400.010.0089181
CTACCGT53350.09.96633158
CGGGGTT129200.09.8310131
CGCGGGT73650.09.4303891
CGGGAAT95300.08.7855571
CTCGGAT112750.08.6916091
AAGGGGG385650.08.48366782-83
CTCGAAT83200.08.3479351
CGGGATT65650.08.3332161
CTCGGGT104300.08.0730661
CGCGTAT8259.566134E-68.0727891
CTCGGTT71600.07.8400441
CGGGTAT43300.07.80045561
TAGGGGG130250.07.671903650-51
CTGGAAT403600.07.6614631
ACTACCG68900.07.64767177
AAAAGGG434500.07.62783280-81
CCGGGAT112100.07.59620951
Produced by FastQC (version 0.11.5)