FastQCFastQC Report
Fri 7 Dec 2018
HGHK3BGX9_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHK3BGX9_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17036662
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1127270.6616730436983489No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC178150.035.7237558
TCGGAAG181100.034.5671163
CGGAAGA183650.034.3712354
ATCGGAA184750.034.3130342
GATCGGA182600.033.1295171
CGCGAAA30550.031.48901236-37
CCGCGAA31850.029.15799734-35
TCCGCGA31750.028.65137134-35
CACGTCT130650.027.97145514-15
CGGCTAT44700.027.89601134-35
TCACCGG43750.027.3031530-31
CGTCTGA134600.027.11543516-17
TCTCGTA73850.027.01445642-43
CACACGT137300.026.99740612-13
CCGGCTA45550.026.8516132-33
CTCGTAT69150.026.8227544-45
ACCGGCT44800.026.45294432-33
ACTCCGC32850.026.31585732-33
GCGAAAT36300.026.3047836-37
GTCACCG44700.026.03299328-29
Produced by FastQC (version 0.11.5)