FastQCFastQC Report
Fri 7 Dec 2018
HGHK3BGX9_n01_AHm30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHK3BGX9_n01_AHm30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21024966
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT758260.360647432200366TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA122250.039.935136-37
TATGCCG103600.038.3307248-49
CGTATGC103950.037.8390646-47
CTCGTAT105750.036.81403744-45
TCGTATG100450.035.49323744-45
TCCGCGA144750.033.83996234-35
CGAAATC142400.033.3667938-39
GCGAAAT138550.033.059236-37
CCGCGAA137750.033.041834-35
ACTCCGC154800.031.65543732-33
ATCTCGT133000.030.57742-43
AATCTCG126850.030.48941240-41
TCTCGTA121550.030.2132442-43
CACTCCG163550.027.95572730-31
CTCCGCG180900.025.55196632-33
ATGCCGT142100.025.471948-49
CCGTCTT152200.025.12272352-53
GTATGCC151800.025.05094346-47
AATCGCG12900.025.04576740-41
ATCGGAA372900.024.5827982
Produced by FastQC (version 0.11.5)