FastQCFastQC Report
Fri 7 Dec 2018
HGHK3BGX9_n01_AHm29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHK3BGX9_n01_AHm29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19160888
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT1360750.710170635097914TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA213850.043.30405436-37
CGTATGC175700.041.8113546-47
TATGCCG178350.041.52075248-49
CTCGTAT177850.040.68014544-45
TCCGCGA235350.039.62850634-35
TCGTATG168450.039.3404844-45
CGAAATC227350.039.0921338-39
ACTCCGC243700.038.266832-33
CCGCGAA228200.037.71588534-35
GCGAAAT227500.037.49989336-37
ATCTCGT202250.037.125842-43
GAGCACA477500.036.3971259
AGAGCAC477450.036.2720838
CGCGTAT13500.036.0683744-45
ATCGGAA476100.036.044672
TCGGAAG481600.035.721793
TCTCGTA191400.035.63125642-43
AATCTCG199600.035.52771840-41
GATCGGA486950.035.11511
CGGAAGA492700.034.9175644
Produced by FastQC (version 0.11.5)