FastQCFastQC Report
Fri 7 Dec 2018
HGHK3BGX9_n01_AHm28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHK3BGX9_n01_AHm28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18309204
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA20300.013.102939
CGCGAAA16550.011.05371336-37
CTACCGT26400.010.4353928
ACTACCG31300.010.1675517
CGCGAAT9357.912604E-109.6520511
CGTATGC16600.09.01434246-47
CGTATCG22100.08.8117243
CGCGGGT38150.08.7152761
GATCGGA195200.08.39446894-95
CGGGAAT41550.08.345061
ATCGGAA186800.08.19986594-95
CGCTAAT9357.2203693E-78.1280431
CGACCGT15203.6379788E-128.1247011
CCGGGAT51200.08.07099151
CGTATGG19200.08.03984812-13
AGATCGG222650.07.999492694-95
GGGGAAT87850.07.83977941
CGGAAAT40300.07.778871
CCGTATG20550.07.742992410-11
CTCGGTT35000.07.59971951
Produced by FastQC (version 0.11.5)