FastQCFastQC Report
Fri 7 Dec 2018
HGHK3BGX9_n01_AHm27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHK3BGX9_n01_AHm27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35401299
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT1958550.5532424106810317TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA311100.042.18299536-37
TATGCCG256900.041.1209648-49
CGTATGC258950.040.73441346-47
CTCGTAT261600.039.72410644-45
TCGTATG249150.037.9529144-45
TCCGCGA349650.037.7068734-35
CGAAATC339200.037.5331838-39
CCGCGAA337250.035.8883334-35
ACTCCGC368150.035.86073732-33
GCGAAAT337600.035.7826336-37
ATCTCGT312100.034.9498942-43
TCTCGTA285900.034.00676342-43
AATCTCG305550.033.3544840-41
ATCGGAA751050.032.316462
TCGGAAG767000.031.8053463
GAGCACA773100.031.7577679
CGCGTAT20850.031.67032644-45
CACTCCG385600.031.59866130-31
AGAGCAC775800.031.5862838
GATCGGA772150.031.3603951
Produced by FastQC (version 0.11.5)