FastQCFastQC Report
Fri 7 Dec 2018
HGHK3BGX9_n01_AHm26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHK3BGX9_n01_AHm26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26850397
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT2129300.7930236562237795TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA323900.043.36250336-37
CGTATGC265350.042.285546-47
TATGCCG268200.042.12539348-49
CTCGTAT267400.041.63390744-45
TCGTATG252600.040.0392544-45
CGAAATC339900.039.93797338-39
TCCGCGA354300.039.7797234-35
ATCTCGT301400.038.54747842-43
ACTCCGC366250.038.53001832-33
GCGAAAT344750.037.76994736-37
CCGCGAA346550.037.55040734-35
AATCTCG296600.036.85254740-41
TCTCGTA285150.036.72892842-43
GAGCACA736050.035.9438979
AGAGCAC747300.035.3206258
CACTCCG380900.034.2765630-31
ATCGGAA774750.033.8290142
TCGGAAG781600.033.5689963
CCGTCTT322950.033.33485452-53
CTCCGCG392750.033.2631832-33
Produced by FastQC (version 0.11.5)