FastQCFastQC Report
Fri 7 Dec 2018
HGHK3BGX9_n01_AHm25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHK3BGX9_n01_AHm25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14381104
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT5872704.0836225090924865TruSeq Adapter, Index 6 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTA496870.3455019864956126TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCGCGTAT339360.23597632003773839TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTTT166030.11545010730747791TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA1146800.065.031239
AGAGCAC1169200.063.805648
CGGAAGA1192250.062.5194174
TCGGAAG1202800.061.917773
AAGAGCA1210600.061.627337
ATCGGAA1214150.061.253112
GAAGAGC1222300.061.007196
GATCGGA1231100.060.2536771
GGAAGAG1312400.056.883435
CGTATGC702550.046.22005546-47
CTCGTAT700150.046.12849444-45
CGCGAAA874650.046.10027736-37
TATGCCG711850.045.7956748-49
TCCGCGA887150.045.5142734-35
ATCTCGT737450.045.41775542-43
CGAAATC861450.045.1439138-39
ACTCCGC905350.044.61549432-33
TGCCGTC702150.044.21447450-51
CCGTCTT693700.044.20047452-53
CGCGTAT42250.044.07644744-45
Produced by FastQC (version 0.11.5)