FastQCFastQC Report
Fri 7 Dec 2018
HGHK3BGX9_n01_AHm24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHK3BGX9_n01_AHm24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10681431
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT1980751.8543863645236298TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTA133510.12499261568978913TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA417350.049.212789
CGGAAGA428550.047.70364
AGAGCAC429900.047.6998
TCGGAAG434150.047.0653043
ATCGGAA441000.046.301932
AAGAGCA450750.045.3973587
CGTATGC241000.045.23605346-47
CTCGTAT235650.045.00422344-45
GATCGGA452950.044.976771
GAAGAGC459800.044.5242776
TATGCCG246150.044.22902748-49
ATCTCGT240950.043.5526242-43
TCGTATG239250.042.6591944-45
CGGCTAT258400.042.13936234-35
TATGATC253000.041.48235338-39
TCTCGTA238250.041.4042142-43
ACCGGCT264200.041.29267532-33
CCGTCTT265000.040.84037452-53
TGCCGTC266850.040.70834750-51
GCCGTCT252700.040.1589350-51
Produced by FastQC (version 0.11.5)