FastQCFastQC Report
Fri 7 Dec 2018
HGHK3BGX9_n01_AHm23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHK3BGX9_n01_AHm23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20916554
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT2248381.0749284992164578TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG284800.044.33120748-49
CGTATGC284900.044.27804646-47
CTCGTAT282400.043.68710344-45
ATCTCGT292100.041.880542-43
TCGTATG283150.041.39868544-45
CGGCTAT314400.040.28634634-35
TCTCGTA287400.039.7469942-43
TCACCGG326150.038.79902330-31
CCGTCTT327550.038.4858252-53
TGCCGTC333400.037.96039250-51
TATGATC325600.037.70714638-39
GCCGTCT312300.037.59733250-51
GAGCACA639800.037.023049
ATGCCGT318850.036.97514748-49
GTATGCC319000.036.8718246-47
ACCGGCT346150.036.6356432-33
GTCACCG328150.035.75662228-29
ATCGGAA665150.035.297742
AGAGCAC670650.035.199748
GCTATGA363100.034.89190336-37
Produced by FastQC (version 0.11.5)