FastQCFastQC Report
Fri 7 Dec 2018
HGHK3BGX9_n01_AHm22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHK3BGX9_n01_AHm22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38403791
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT1726430.4495467647972566TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC234550.039.5040146-47
TATGCCG237550.039.0623148-49
CTCGTAT235950.038.4051444-45
TCGTATG229900.037.99001344-45
TCTCGTA260950.032.68866742-43
CGGCTAT283350.032.6632334-35
ATCTCGT279300.032.29302642-43
TCACCGG313500.029.72862630-31
CCGGCTA295800.029.65490232-33
ACCGGCT322100.028.98125332-33
TATGATC313700.028.8086638-39
ATGCCGT315150.027.9969748-49
CCGTCTT336300.027.82373252-53
GTATGCC318100.027.7318546-47
GTCACCG320250.027.52279728-29
GCCGTCT330850.026.73192650-51
TGCCGTC361250.025.88925750-51
GAGCACA707750.025.2209019
ATCGGAA697050.025.021112
TCGGAAG716050.024.376933
Produced by FastQC (version 0.11.5)