FastQCFastQC Report
Fri 7 Dec 2018
HGHK3BGX9_n01_AHm21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHK3BGX9_n01_AHm21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21874632
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT2292621.0480724887166102TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG279850.044.1655448-49
CGTATGC282300.043.8100346-47
CTCGTAT278150.043.32845744-45
TCGTATG278900.041.9174644-45
ATCTCGT288850.041.2232842-43
TCTCGTA283000.039.9603242-43
CGGCTAT308750.039.90761634-35
GCCGTCT309850.037.79542550-51
CCGTCTT328050.037.6530852-53
TCACCGG329750.037.6484230-31
TGCCGTC330200.037.47281650-51
GAGCACA631250.037.3670279
GTATGCC314850.037.2289146-47
ATGCCGT315850.037.10136448-49
TATGATC324800.036.7684838-39
ACCGGCT342850.036.20562432-33
ATCGGAA654600.035.6560672
GTCACCG330450.035.50717528-29
AGAGCAC664600.035.4491278
CGGAAGA668300.035.0894744
Produced by FastQC (version 0.11.5)