FastQCFastQC Report
Fri 7 Dec 2018
HGHK3BGX9_n01_AHm20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHK3BGX9_n01_AHm20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22577489
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT1376130.6095141935403002TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC177000.041.38408746-47
TATGCCG177700.041.17886748-49
CTCGTAT178400.039.96879644-45
TCGTATG174450.039.56670444-45
CGGCTAT204200.035.72566234-35
ATCTCGT198500.035.5406842-43
TCTCGTA189700.035.29860342-43
TCACCGG226150.032.46258530-31
CCGGCTA214050.032.21439732-33
ACCGGCT230950.031.84206232-33
TATGATC221950.031.67216938-39
CCGTCTT234300.031.45252852-53
ATGCCGT223850.031.0765248-49
ATCGGAA457450.030.2568992
GTATGCC230850.030.14601946-47
TCGGAAG463350.029.9637533
GAGCACA469300.029.9179869
AGAGCAC470900.029.7057068
GCCGTCT234150.029.6170350-51
GATCGGA470300.029.4002151
Produced by FastQC (version 0.11.5)