FastQCFastQC Report
Fri 7 Dec 2018
HGHK3BGX9_n01_AHm19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHK3BGX9_n01_AHm19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21951244
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT1085580.4945414483115399TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG148600.040.14772848-49
CGTATGC150350.039.7313946-47
CTCGTAT150150.038.96271544-45
TCGTATG146650.038.80742644-45
TCTCGTA161650.034.2970342-43
CGGCTAT178300.033.65472434-35
ATCTCGT173000.033.6533742-43
CCGGCTA185700.030.59688432-33
TATGATC190450.030.54897138-39
TCACCGG198950.030.23922730-31
ACCGGCT200450.030.01629332-33
ATCGGAA384600.029.4796872
ATGCCGT196250.029.18957548-49
CCGTCTT206450.029.16126852-53
GTATGCC198900.028.70773346-47
GATCGGA395700.028.593241
TCGGAAG399550.028.5190413
GTCACCG203050.028.1673728-29
GCCGTCT203450.028.13245450-51
AGAGCAC413900.028.0130278
Produced by FastQC (version 0.11.5)