FastQCFastQC Report
Fri 7 Dec 2018
HGHK3BGX9_n01_AHm18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHK3BGX9_n01_AHm18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23117509
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT869990.37633379963213165TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC121500.038.68664646-47
TATGCCG121700.038.4839648-49
TCGTATG119750.037.34888544-45
CTCGTAT125650.036.57811444-45
CGGCTAT148550.031.82199534-35
TCTCGTA138600.031.56834242-43
ATCTCGT149850.030.59327342-43
TCACCGG166950.028.43837730-31
CCGGCTA160100.028.02501532-33
ACCGGCT174700.027.1920732-33
ATGCCGT169250.026.46527348-49
GTATGCC170700.026.43696846-47
TATGATC174450.026.2825338-39
CCGTCTT183950.025.84720452-53
GTCACCG175300.025.67538528-29
GCCGTCT180950.025.05529250-51
ATCGGAA365900.024.39152
TCGGAAG378100.023.780343
GATCGGA376350.023.6390171
TGCCGTC202100.023.59660350-51
Produced by FastQC (version 0.11.5)