FastQCFastQC Report
Fri 7 Dec 2018
HGHK3BGX9_n01_AHm17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHK3BGX9_n01_AHm17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39669932
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT1316450.33185083352298156TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG187150.037.3857948-49
CGTATGC188400.037.2659446-47
TCGTATG182350.036.90102444-45
CTCGTAT191900.035.54733744-45
TCTCGTA216100.030.2936942-43
CGGCTAT233500.029.93057334-35
ATCTCGT233500.029.20613342-43
CCGGCTA250700.026.79419932-33
TCACCGG267450.026.57051330-31
ACCGGCT275100.025.64375132-33
ATGCCGT268900.025.26915648-49
TATGATC273550.024.93363238-39
GTATGCC273150.024.86872146-47
CCGTCTT287100.024.57631352-53
GTCACCG280550.024.24665828-29
GCCGTCT288700.023.58486450-51
TGCCGTC322200.022.04668850-51
ATCGGAA619850.021.5477222
GATCTCG313800.021.09897240-41
GCTATGA340100.020.93463336-37
Produced by FastQC (version 0.11.5)