FastQCFastQC Report
Fri 4 Jan 2019
HGHK2BGX9_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHK2BGX9_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26894864
Sequences flagged as poor quality0
Sequence length49
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23985948.918409105917027No Hit
GACTGGGAGGGTATTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1705220.6340318359668968No Hit
GACTGGGAGGGTATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1619600.6021967614337073No Hit
GACTGGGAGGGTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA905240.3365847100026236No Hit
AAGTATCGTTTCGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA457810.1702220914744168No Hit
TTCCGCCTCTCTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA453650.1686753277503095No Hit
GCATTCTGTCACCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA421480.15671393616268145No Hit
GCATTCTGTCACCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA412580.1534047541567788No Hit
CTCTATTCAGACCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA322980.12008984317600564No Hit
GCATTCTGTCACCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA318120.11828280670986105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAAAC1430450.042.5298913
CGAAACA1418750.042.46323414
AGGACGA1478800.042.4305510
AACACCG1421150.042.4089617
ACACCGG394050.041.99820718
ACACCGA410000.041.9850318
TTGTGGA1505950.041.9714932
CGTTTCG168450.041.9461147
ACACCGC390000.041.8719318
ACTGGGA880350.041.6987462
TTTCGCA172300.041.609349
GGACGAA1487850.041.45718411
ACACCGT283600.041.38512418
GACTGGG889650.041.2162741
TGTGGAA1549050.041.11273
GACGAAA1473450.041.0944812
GGTATTC188200.041.0397410
GTCCGAC57800.040.9197124
GGGTATT911150.040.9090549
GTGGAAA1570950.040.8321534