FastQCFastQC Report
Fri 4 Jan 2019
HGHK2BGX9_n02_cDNA3P_d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHK2BGX9_n02_cDNA3P_d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86112633
Sequences flagged as poor quality0
Sequence length49
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9005741.0458093878049228No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1585850.034.4626431
AGCAGTG1796900.030.353022
TCAACGC1912300.027.52387812
CAACGCA1922900.027.34427613
ATCAACG1939150.027.33566511
GTGGTAT1985350.027.2502236
AGTGGTA2024300.026.7985175
TATCAAC1996700.026.47699410
CGCAGAG2027000.026.00620816
GAGTACA2000150.025.9947320
GTACATG2006800.025.93521322
CAGTGGT2110250.025.7768884
GTATCAA2068050.025.699829
TGGTATC2101600.025.674617
AGAGTAC2039150.025.55477519
TACATGG2038200.025.54907823
GGTATCA2109800.025.492338
AGTACAT2029500.025.4501521
AACGCAG2086950.025.39277514
GCAGAGT2074550.025.2464717
Produced by FastQC (version 0.11.5)