FastQCFastQC Report
Fri 4 Jan 2019
HGHK2BGX9_n02_cDNA3P_c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHK2BGX9_n02_cDNA3P_c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81872878
Sequences flagged as poor quality0
Sequence length49
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8054440.9837738939627847No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1541500.034.3973541
AGCAGTG1724550.030.612382
TCAACGC1858750.027.64591412
ATCAACG1880000.027.49122211
CAACGCA1875150.027.39963713
GTGGTAT1922050.027.3658056
AGTGGTA1956100.026.948115
TATCAAC1947100.026.58951610
GAGTACA1938300.026.16966820
CGCAGAG1959150.026.14516416
CAGTGGT2028350.026.0165214
GTACATG1950900.025.9782622
GTATCAA2014450.025.8340639
TGGTATC2037600.025.8025827
GGTATCA2038200.025.7179898
AGAGTAC1980300.025.712619
AGTACAT1972550.025.62889521
TACATGG1980300.025.59768123
AACGCAG2033600.025.38119114
GCAGAGT2013200.025.3684717
Produced by FastQC (version 0.11.5)